The Possible Protective Role of Hesperidin Versus Acrylamide-Induced Neurotoxic Effects on the Prefrontal Cortex of The Adult Male Albino Rat

Document Type : Original Article

Authors

Department of Human Anatomy and Embryology, Faculty of Medicine, Assiut University, Egypt.

Abstract

Background: Numerous harmful consequences appeared-with appearance of acrylamide (AC) in our daily consuming products. Hesperidin (Hs) is a flavone glycoside that is commonly used as anti-inflammatory agent. Aim of the Work: to offer an experimental foundation for prevention and reduction of acrylamide poisoning using hesperidin. Material and Methods: 40 adult male albino rats were divided into four groups: Control group (I), Hs treated group (II) with a dose of 10 mg/ kg B. W. /day. Hs was dissolved in 0.1% carboxy methyl cellulose, AC treated group (III) with a dose of 25 mg/kg B. W. /day and AC-Hs group (IV) received the same doses as Group II and Group III. All treatments were given orally via gavage for 21- consecutive days. At the end of experiment, prefrontal cortices were extracted and processed for the light, electron microscopic examination and immunohistochemical staining. Morphometric measurements were also done. Results: AC treated group revealed hemorrhage and congestion in both cortical blood vessels and pia mater. Pyramidal cells were shrunken with pyknotic nuclei, pericellular halos and loss of their processes. The granule cells were affected and showed ill-defined boundaries and lost their nuclei. The morphometric studies revealed a significant decrease in body weight and mPFC thickness of AC treated rats. On the other hand, rats received hesperidin with acrylamide revealed more or less apparent normal architecture as control group. Conclusion: Treatment with hesperidin is able to attenuate acrylamide induced brain damage in the rats.

Keywords

Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry
Volume 17, Issue 2
December 2025
Pages 1-20

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