Evaluation of the Protective Role of Intravesical Chondroitin Sulfate Versus Intravesical Dimethyl Sulfoxide on The Glycosaminoglycan's, Uroplakin and Restoration of Barrier Function of the Urinary Bladder in A Rat model of Ketamine Induced Interstitial Cystitis. (Histological and Immunohistochemical Study)

Document Type : Original Article

Author

Department of Anatomy and Embryology. Faculty of Medicine, Ain-Shams University. Abbassia, Postal code: 11591 Cairo, Egypt.

Abstract

Ketamine recently used as a treatment of resistant depression and bipolar disorder. It has been noted that chronic use of ketamine may induce cystitis and urological toxicity. The purpose of the present study is to examine the protective role of intravesical chondroitin sulphate versus DMSO against ketamine effect on the urinary bladder. Twenty- eight adult albino rats divided into 4 groups seven rats per each: Group (I) received 1ml saline by intraperitoneal injection. Group (II) received 30mg/kg/d ketamine by intraperitoneal injection. Group (III) received 30 mg/kg/d ketamine + intravesical 1ml DMSO 50%. Group (IV) received 30 mg/kg/d ketamine + intravesical 1 mL of 2% chondroitin sulfate. Group (Ⅱ) showed areas of urothelial cell degeneration and surface ulcerations. Mast cells appeared in the lamina propria and all layers of the urothelium. Marked distortion of the superficial umbrella cells, wide cracks appeared separating them from each other. There was significant increase in IL8 and UPIII expression in group II in comparison to group I with (P value<0.5). Group III showed restoration of normal shape of the superficial cells in some areas; with persistence of surface cracking. Furthermore, there was significant decrease in IL8 and UPIII expression in group III in comparison to group II with (P value<0.5). The best results observed in group IV. Dimethyl sulfoxide (DMSO) induced moderate improvement on the bladder urothelium and decreased inflammation. Chondroitin sulfate rendered a remarkable improvement via its strong anti-inflammatory effect

Keywords

Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry
Volume 14, Issue 2
December 2022
Pages 201-214

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