Copper Oxide Nanoparticles Stimulate Cellular Damage and Histological Architecture Deterioration in Tissues of Culex Pipiens Larvae (Diptera: Culicidae).

Ragheb, Mona; Soliman, Mohamed I.; Taha, Riham H.

doi:10.21608/eajbsd.2022.249341

Copper Oxide Nanoparticles Stimulate Cellular Damage and Histological Architecture Deterioration in Tissues of Culex Pipiens Larvae (Diptera: Culicidae).

Document Type : Original Article

Authors

General Organization of Teaching Hospitals and Institutes (GOTHI), Research Institute of Medical Entomology (RIME), P.O. Box 12311 Dokki, Cairo, Egypt

10.21608/eajbsd.2022.249341

Abstract

Culex pipiens, is one of the considerable vector-borne diseases widely distributed all over the world. The extensive use of synthetic insecticides caused various human health hazardous, insect resistance and environmental pollution. As an alternative control strategy, nanoparticle applications in insect management are mandatory. Mainly, the aim of this current study is to elucidate the toxic effect of copper oxide nanoparticles (CuONPs) on larval tissues of Culex pipiens regarding the histological and cellular damage aspects. The lethal concentrations LC₁₀, LC₂₅, LC₅₀ and LC₉₀ were determined from the established regression log concentrate-response lines after 24 hours of treatment. Four replicates were considered for each concentration where twenty-five third instar larvae were involved for each replicate. Results revealed significant increase in mortality rate upon increasing copper oxide nanoparticles (CuONPs) concentrations which recorded 0.040, 0.099, 0.268 and 1.767 mg/ ml for LC₁₀, LC ₂₅, LC_50, and LC₉₀ respectively. Data in this study showed a significant increase in cellular damage enzyme levels namely, lipid peroxidase and nitric oxide, and a significant decrease in levels of total protein while, an increase in albumin proportions were detected in tissue homogenates of treated larvae of the increasing mentioned lethal CuONPs concentrations. Meanwhile, histological studies implied severe deterioration in tissue architecture of the treated larvae which increases markedly as the CuONPs concentration increase as well. In conclusion, CuONPs increase the cellular damage and deteriorate the histological structure of tissues of Culex pipiens larvae which render them promising and smart larvicidal agents.

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