Comparison of neutrophil elastase levels in obese and normal individuals

Document Type : Original Article

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College of Applied Medical Sciences, University of Ha’il, Ha’il-2440, Saudi Arabia

Abstract

Purpose: Neutrophils being the most abundant leukocytes in humans and the first cells to arrive on the site of inflammatory immune response. These cells are classically character­ized by their ability to act as phagocytic cells, to release lytic enzymes like neutrophil elastase from azurophil granules. Neutrophil elastase (NE) is known to promote inflammatory responses in several disease. The purpose of this study is to correlate neutrophil elastase expression level with obesity.
Method: Present study aims to determine the differential expression and packaging (in azurophil granules of neutrophils) of human NE using Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) in normal and obese individuals.
Results: It was observed that, the obese individuals tend to have an average elevated levels neutrophil elastase in blood neutrophils as confirmed by the bands observed in SDS-PAGE. The elevated levels of neutrophil elastase in obese individuals may be attributed to chronic inflammation in fat tissue.   
Conclusion: Obese individuals (Students of Hail University) have a slightly higher level of neutrophil elastase in blood leukocyte (neutrophils).

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